circRNA basic information
circBase ID: hsa_circ_0001417
Name: hsa_circ_ANKRD17
Synonym: circ ankyrin repeat domain 17
Host Gene: ANKRD17
Genomic location(hg19): chr4:73950965-73958017:-
Genomic location(hg38): chr4:73085248-73092300:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BC tissues; xenografts
Cell lines:

SK-BR-3; BT-549; MCF-10A; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: hexokinase 2 / HK2, Ago2 protein
Associated microRNA: miR-143
Biological function: Promotes glycolysis, cell growth, migration, invasion, cell-cycle progression, glucose consumption, lactate production, 18 F-FDG uptake, and xenograft tumor growth in breast cancer cells.
Molecular mechanism: circANKRD17 functions as a ceRNA/miRNA sponge for miR-143, relieving miR-143-mediated repression of HK2 and thereby promoting glycolysis in breast cancer cells.
Biological pathway or process:

glycolysis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); ceRNA regulation (promotes)
Detected method:
Q
H
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Cell Cycle Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis
Clinical significance:

High circANKRD17 expression in BC patients was associated with higher SUV max on 18 F-FDG PET/CT, and circANKRD17 was suggested as a promising therapeutic target to interrupt cancerous glycolysis.
Description:

circANKRD17 is up-regulated in breast cancer cells and promotes glycolysis and malignant phenotypes including proliferation, migration, invasion, and cell-cycle progression. Mechanistically, circANKRD17 acts as a cytoplasmic sponge for miR-143, relieving repression of HK2 and increasing glycolytic activity, including glucose consumption, lactate production, and 18 F-FDG uptake. In patient BC tissues, high circANKRD17 expression is associated with higher SUV max on 18 F-FDG PET/CT, supporting its potential as a therapeutic target for cancerous glycolysis.
Confidence score:

0.8793
Other information
Title:

CircANKRD17 promotes glycolysis by inhibiting miR-143 in breast cancer cells.

Journal: Journal of cellular physiology
Published: 2023
PubMed ID: 37812578
Study type:

combined biological and clinical study
Data availability: Supporting Information
Code availability: -