| Expression pattern: |
UP |
| Associated gene: |
PLAG1, ATG12 |
| Associated microRNA: |
miR-488-3p |
| Biological function: |
promotes proliferation, migration, invasion and autophagy-associated cisplatin chemoresistance; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA sponging of miR-488-3p leading to activation of PLAG1 and ATG12 |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); autophagy (promotes); chemoresistance (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
RT-qPCR; ISH (In Situ Hybridization); FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; MTT; Annexin V/PI Flow Cytometry; IF (Immunofluorescence); Western Blot; ChIP / ChIP-seq; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis; Survival Analysis |
| Clinical significance: |
increased circPOFUT1 expression indicated poor prognosis |
| Description: |
circPOFUT1 is upregulated in gastric cancer and is associated with worse patient survival. It promotes proliferation, migration, invasion, and autophagy-associated cisplatin resistance by sponging miR-488-3p to activate the PLAG1-driven ATG12 program, enhancing tumor growth in xenografts. |
| Confidence score: |
0.8772 |