| Expression pattern: |
UP |
| Associated gene: |
RAI14, Ago2 |
| Associated microRNA: |
miR-23b-3p |
| Biological function: |
Promotes GC cell growth, colony formation, invasion and in vivo tumorigenesis; circNFATC3 knockdown inhibits these phenotypes. |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-23b-3p, thereby upregulating RAI14; associated with Ago2-containing RISC. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); metastasis (other); ceRNA regulation (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
RT-qPCR; Microarray; RNase R Treatment; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circNFATC3 expression is associated with poor prognosis (shorter overall survival) in patients with GC. |
| Description: |
In gastric cancer, circNFATC3 (hsa_circ_0039930) is up-regulated and mainly localized in the cytoplasm. It promotes tumor growth and invasion by sponging miR-23b-3p to increase RAI14, and high circNFATC3 expression is linked to poorer overall survival. |
| Confidence score: |
0.8222 |