| Expression pattern: |
UP |
| Associated gene: |
PML mRNA, PML |
| Associated microRNA: |
- |
| Biological function: |
Promotes AML cell proliferation and AML development, inhibits apoptosis, enhances leukemia cell proliferation and infiltration in vivo, and reduces recipient mouse survival when expressed. |
| Molecular mechanism: |
circAFF2 directly binds the 3′UTR of PML mRNA, promotes PML mRNA degradation, reduces PML expression, and thereby regulates AML cell proliferation and apoptosis. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); mRNA stability (inhibits); other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; H&E Staining; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circAFF2 may provide potential targets or strategies for clinical diagnosis and treatment of AML. |
| Description: |
circAFF2 is a human AFF2-derived circRNA that is significantly upregulated in AML patients and AML cell lines. It promotes AML cell proliferation and suppresses apoptosis by directly binding the 3′UTR of PML mRNA, accelerating PML mRNA degradation and reducing PML tumor suppressor expression. Knockdown of circAFF2 alleviates AML progression in a cell line-derived xenograft model and prolongs mouse survival. |
| Confidence score: |
0.8416 |