| Expression pattern: |
UP |
| Associated gene: |
IKK-beta, AGO2, NF-kappaB signaling pathway |
| Associated microRNA: |
miR-214-3p |
| Biological function: |
Promotes cigarette smoke/CSE-induced lung inflammation and inflammatory cytokine (CXCL1, IL-6) expression; circFOXO3 knockdown attenuates inflammatory cell infiltration (macrophages, neutrophils) and lung injury. |
| Molecular mechanism: |
circFOXO3 acts as a miR-214-3p sponge, relieving repression of IKK-beta, thereby activating NF-kappaB signaling (IkappaBalpha and p65 phosphorylation; p65 nuclear translocation) and enhancing inflammatory cytokine expression. |
| Biological pathway or process: |
NF-kappaB (promotes); inflammation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; IF (Immunofluorescence); ELISA; H&E Staining; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
circFOXO3 might be a therapeutic target for CS-induced inflammatory disorders/COPD-related inflammation. |
| Description: |
circFOXO3 is up-regulated in cigarette smoke-exposed mouse lungs and CSE-treated MLE12 cells and is mainly cytoplasmic. It promotes lung inflammation by sponging miR-214-3p to increase IKK-beta expression, thereby activating NF-kappaB signaling and increasing inflammatory cytokines (e.g., CXCL1, IL-6); circFOXO3 knockdown ameliorates CS-induced lung injury/inflammation. |
| Confidence score: |
0.7223 |