| Expression pattern: |
UP |
| Associated gene: |
MAP7, AKT3, P-AKT |
| Associated microRNA: |
miR-16 |
| Biological function: |
promotes cell proliferation; promotes migration; inhibits apoptosis |
| Molecular mechanism: |
circNF1 acts as a miR-16 sponge, derepressing MAP7 and AKT3 and increasing AKT phosphorylation. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); apoptosis (inhibits); PI3K/AKT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; Back-Splice Junction PCR / divergent primers PCR; divergent primers PCR; Sanger Sequencing; RNase R Treatment; RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; Western Blot; CCK8; Colony Formation Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Bioinformatics Analysis |
| Clinical significance: |
circNF1 represents a novel potential biomarker and therapeutic target in GC patients. |
| Description: |
circNF1 (hsa_circ_0042881), derived from NF1 exons 2-8, is up-regulated in gastric carcinoma tissues and GC cell lines. It promotes malignant phenotypes (proliferation and migration) and reduces apoptosis by sponging miR-16, thereby increasing MAP7 and AKT3 translation and enhancing AKT pathway activity. |
| Confidence score: |
0.8052 |