circRNA basic information
circBase ID: -
Name: mmu_circ_CDR1AS
Synonym: circ-CDR1as
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0002679
MONDO name: cerebral infarction
Disease details: cerebral ischemia
Disease DO ID:
 3526
Disease MeSH ID:
 D002544
Disease NCIt ID:
 C50486
Disease ICD11 ID:
 1614716959; 636274910
Disease OMIM ID:
 -
Species: Mouse
Species details: Mus musculus
Tissue specimen:

-
Cell lines:

HT-22
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: TRAF3
Associated microRNA: miR-28-3p
Biological function: inhibits proliferation; promotes apoptosis; promotes inflammatory responses; aggravates H/R-induced neuronal dysfunction; weakens DEX neuroprotection
Molecular mechanism: ceRNA mechanism: circ-CDR1as sponges miR-28-3p to positively regulate TRAF3 expression
Biological pathway or process:

proliferation (inhibits); apoptosis (promotes); inflammation (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Transfection; CCK8; Annexin V/PI Flow Cytometry; ELISA; Luciferase Reporter Assay; Bioinformatics Analysis
Clinical significance:

-
Description:

In a CI/R in-vitro model (H/R-treated HT-22 hippocampal neurons), circ-CDR1as is induced by H/R but reduced by dexmedetomidine (DEX). Functionally, circ-CDR1as promotes neuronal injury phenotypes (reduced viability, increased apoptosis and inflammatory cytokines) by sponging miR-28-3p to upregulate TRAF3, thereby counteracting DEX-mediated neuroprotection.
Confidence score:

0.5554
Other information
Title:

Circular RNA cerebellar degeneration-related protein 1 antisense RNA (Circ-CDR1as) downregulation induced by dexmedetomidine treatment protects hippocampal neurons against hypoxia/reoxygenation injury through the microRNA-28-3p (miR-28-3p)/tumor necrosis factor receptor-associated factor-3 (TRAF3) axis.

Journal: Bioengineered
Published: 2021
PubMed ID: 34787053
Study type:

biological research
Data availability: -
Code availability: -