circRNA basic information
circBase ID: -
Name: hsa_circ_ITCH
Synonym: -
Host Gene: ITCH
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008903
MONDO name: lung cancer
Disease details: lung cancer
Disease DO ID:
 1324
Disease MeSH ID:
 -
Disease NCIt ID:
 C7377
Disease ICD11 ID:
 -
Disease OMIM ID:
 211980
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; adjacent noncancer tissues
Cell lines:

A549; NCI-H460
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: ITCH, beta-catenin, TCF, c-Myc, cyclinD1
Associated microRNA: miR-7, miR-214
Biological function: Inhibits lung cancer cell proliferation.
Molecular mechanism: Acts as a miRNA sponge (ceRNA-like), binding miR-7 and miR-214 to increase ITCH expression, thereby inhibiting Wnt/beta-catenin signaling (reduced beta-catenin/TCF activity and downstream c-Myc/cyclinD1).
Biological pathway or process:

Wnt/beta-catenin (inhibits); proliferation (inhibits); cell cycle (inhibits)
Detected method:
Q
Validation methods:

divergent primers PCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Clinical Sample Validation; Actinomycin D / DRB Stability Assay; Luciferase Reporter Assay; Transfection; Western Blot; CCK8; Bioinformatics Analysis
Clinical significance:

-
Description:

cir-ITCH is down-regulated in lung cancer tissues. It suppresses lung cancer cell proliferation by sponging miR-7 and miR-214, increasing ITCH expression and inhibiting Wnt/beta-catenin signaling (reduced beta-catenin/TCF activity and downstream c-Myc/cyclinD1).
Confidence score:

0.7769
Other information
Title:

Circular RNA-ITCH Suppresses Lung Cancer Proliferation via Inhibiting the Wnt/beta-Catenin Pathway.

Journal: BioMed research international
Published: 2016
PubMed ID: 27642589
Study type:

combined biological and clinical study
Data availability: -
Code availability: -