| Expression pattern: |
UP |
| Associated gene: |
CTBP2, E-cadherin |
| Associated microRNA: |
miR-556-5p |
| Biological function: |
promotes proliferation, migration, invasion and metastasis of colorectal cancer cells; promotes tumor growth in vivo |
| Molecular mechanism: |
circHERC4 binds and inhibits miR-556-5p, relieving repression of CTBP2, which suppresses E-cadherin and promotes metastasis (CTBP2/E-cadherin pathway; linked to Notch signaling changes). |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); Notch (other); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; Transwell Assay; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Survival Analysis; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
High expression of circHERC4 was positively associated with lymph node metastasis, distant metastasis and advanced tumor grade, and associated with worse overall survival in CRC patients. |
| Description: |
circHERC4 (hsa_circ_0007113), derived from the HERC4 gene, is up-regulated in colorectal cancer and promotes proliferation and metastasis. It functions mainly in the cytoplasm by binding/inhibiting miR-556-5p, thereby increasing CTBP2 and suppressing E-cadherin, which facilitates metastatic progression; high circHERC4 indicates poor prognosis. |
| Confidence score: |
0.8831 |