| Expression pattern: |
UP |
| Associated gene: |
YAP / YAP1 |
| Associated microRNA: |
miR-515-5p |
| Biological function: |
Promotes osteosarcoma cell proliferation and viability, inhibits apoptosis, promotes invasion/migration, promotes EMT-like changes, and enhances cancer stemness (sphere formation, CD133+CD44+ population, ALDH1/Nanog). |
| Molecular mechanism: |
Acts as a miRNA sponge/ceRNA to bind miR-515-5p, thereby increasing YAP/YAP1 expression; this circPIP5K1A-miR-515-5p-YAP axis regulates osteosarcoma stemness. |
| Biological pathway or process: |
stemness (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; MTT; Annexin V/PI Flow Cytometry; Transwell Assay; Colony Formation Assay; Luciferase Reporter Assay; Western Blot; IF (Immunofluorescence); In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
circPIP5K1A is up-regulated in osteosarcoma tissues and promotes malignant phenotypes including proliferation, invasion/migration, EMT-like changes, and cancer stemness. Mechanistically, it functions through a ceRNA axis by sponging miR-515-5p to increase YAP/YAP1, and its knockdown suppresses tumor growth in a nude mouse xenograft model. |
| Confidence score: |
0.6287 |