circRNA basic information
circBase ID: hsa_circ_0020397
Name: hsa_circ_DOCK1
Synonym: circDOCK1-1
Host Gene: DOCK1
Genomic location(hg19): chr10:128768965-128926028:+
Genomic location(hg38): chr10:126970701-127127764:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
1612
Disease MeSH ID:
-
Disease NCIt ID:
C9335
Disease ICD11 ID:
1047754165
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

breast cancer specimens

Cell lines:

HMLE; mesHMLE; MDA-MB-231; MDA-MB-157; CAMA-1; SKBR-3; HCC-202; HCC-1419; MDA-MB-361; MDA-MB-175VII; ZR75.1; HCC-1500; MCF7; T47D; MDA-MB-436

In vivo animal model:

-

circRNA-disease information
Expression pattern:
DS
Associated gene: AGR2, ENPP1, PPP1R9A, APOE, AQP3, CD99L2, IGFBP4
Associated microRNA: hsa-mir-539-3p, hsa-miR-545-3p
Biological function: promotes epithelial differentiation; increases cell-to-cell adhesion; inhibits growth/proliferation; reduces random and directional motility
Molecular mechanism: Transcriptomic regulation of ~110 common genes; proposed miRNA sponging affecting AGR2/ENPP1/PPP1R9A (in silico prediction, not functionally confirmed).
Biological pathway or process:

proliferation (inhibits); migration (inhibits); EMT (inhibits); ceRNA regulation (other)

Detected method:
Q
Validation methods:

RT-qPCR; RNA-seq; Bioinformatics Analysis; Transfection; MTT; Wound Healing Assay; Western Blot

Clinical significance:

-

Description:

circDOCK1-1 (hsa_circ_0020397), derived from DOCK1, is high in luminal/epithelial breast cancer cell lines but low/undetectable in triple-negative mesenchymal lines. Overexpression promotes epithelial differentiation and cell-cell adhesion while inhibiting proliferation and motility, accompanied by transcriptomic changes in a common set of genes; a miRNA-sponge ceRNA mechanism is proposed in silico (e.g., involving hsa-mir-539-3p and hsa-miR-545-3p) but not functionally confirmed.

Confidence score:

0.5008

Other information
Title:

A DOCK1 Gene-Derived Circular RNA Is Highly Expressed in Luminal Mammary Tumours and Is Involved in the Epithelial Differentiation, Growth, and Motility of Breast Cancer Cells.

Journal: Cancers
Published: 2021
PubMed ID: 34771489
Study type:

biological research

Data availability: E-MTAB-10819
Code availability: -