| Expression pattern: |
UP |
| Associated gene: |
mTOR, E2F1 |
| Associated microRNA: |
- |
| Biological function: |
attenuates BBB damage; maintains endothelial barrier integrity/low permeability; promotes autophagy |
| Molecular mechanism: |
circ-FoxO3 sequesters mTOR and E2F1, inhibits mTORC1 activity, thereby activating autophagy to clear cytotoxic aggregates and protect BBB integrity |
| Biological pathway or process: |
autophagy (promotes); PI3K/AKT/mTOR (inhibits) |
| Detected method: |
Q
H
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Bioinformatics Analysis; Transfection; Western Blot; IF (Immunofluorescence); Tube Formation Assay; In Vivo Animal Model; TEM |
| Clinical significance: |
circ-FoxO3 may be a promising therapeutic target for neurological disorders associated with BBB damage |
| Description: |
circ-FoxO3 is up-regulated in BMECs during cerebral ischemia/reperfusion and hemorrhagic transformation and protects BBB integrity by reducing endothelial barrier permeability. It activates autophagy primarily by sequestering mTOR and E2F1, inhibiting mTORC1 signaling, which helps clear cytotoxic aggregates and attenuate BBB collapse. |
| Confidence score: |
0.7822 |