| Expression pattern: |
UP |
| Associated gene: |
EGR1, HnRNP-L, AGO2 |
| Associated microRNA: |
miR-520h |
| Biological function: |
promotes proliferation, migration, and invasion and induces autophagy in prostate cancer, facilitating tumor growth in vivo |
| Molecular mechanism: |
HnRNP-L upregulates circCSPP1 biogenesis; circCSPP1 acts as a ceRNA (miRNA sponge) for miR-520h to de-repress EGR1, promoting autophagy and malignant phenotypes |
| Biological pathway or process: |
autophagy (promotes); proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
M
|
| Validation methods: |
RT-qPCR; RNA-seq; Microarray; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis; Survival Analysis |
| Clinical significance: |
higher levels of circCSPP1 were associated with the faster biochemical recurrence in patients with prostate cancer |
| Description: |
circCSPP1 is up-regulated in prostate cancer tissues/cells and mainly localizes to the cytoplasm. It is promoted by the RBP HnRNP-L and functions as a ceRNA to sponge miR-520h, thereby increasing EGR1 and inducing autophagy, which enhances proliferation, migration, invasion and xenograft tumor growth; higher circCSPP1 tends to associate with faster biochemical recurrence. |
| Confidence score: |
0.853 |