| Expression pattern: |
UP |
| Associated gene: |
SHP2 |
| Associated microRNA: |
miR-934 |
| Biological function: |
induces CD8+ T cell dysfunction/exhaustion, promotes immunosuppression/immune escape, and promotes resistance to anti-PD1 therapy in NSCLC |
| Molecular mechanism: |
Exosomal circUSP7 acts as a miR-934 sponge in CD8+ T cells, thereby upregulating SHP2 and inducing CD8+ T cell exhaustion/dysfunction, which contributes to anti-PD1 resistance. |
| Biological pathway or process: |
immune regulation (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis; circRIP; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; ELISA; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
High circUSP7 indicates poor clinical prognosis and may serve as a predicted marker of anti-PD1 therapy in NSCLC; plasma exosomal circUSP7 is higher in progressive disease (PD) than in PR/SD after anti-PD1 treatment. |
| Description: |
circUSP7 (hsa_circ_0005152), derived from USP7, is up-regulated in NSCLC tissues and enriched in plasma/cancer cell-derived exosomes. It is transferred to CD8+ T cells where it sponges miR-934, increases SHP2, induces CD8+ T cell dysfunction/exhaustion, and is associated with poor prognosis and anti-PD1 resistance. |
| Confidence score: |
0.8103 |