| Expression pattern: |
UP |
| Associated gene: |
PPP6C |
| Associated microRNA: |
miR-335 |
| Biological function: |
Promotes cervical cancer cell proliferation and inhibits apoptosis; knockdown increases apoptosis and reduces proliferation/cell viability and colony formation; promotes cell cycle progression (knockdown causes cell cycle arrest). |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-335, thereby increasing PPP6C expression; miR-335 inhibition rescues the anti-proliferative effects of circ_103973 knockdown, and si-PPP6C blocks this rescue. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); cell cycle (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Luciferase Reporter Assay; RNA Pull-Down Assay; Survival Analysis |
| Clinical significance: |
Higher levels of circ_103973 were correlated to a worse outcome of CC patients. |
| Description: |
circ_103973 is up-regulated in cervical cancer tissues and cell lines and is associated with worse patient prognosis. Functionally, circ_103973 promotes proliferation and cell-cycle progression and suppresses apoptosis in vitro. Mechanistically, it acts as a ceRNA by sponging miR-335 to increase PPP6C expression (circ_103973/miR-335/PPP6C axis). |
| Confidence score: |
0.7745 |