circRNA basic information
circBase ID: -
Name: hsa_circ_CNOT2
Synonym: circCNOT2 (Chr 12: 70,278,132-70,311,018)
Host Gene: CNOT2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: primary breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

primary breast tumors; plasma
Cell lines:

MCF-7; BT-474
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UN
Associated gene: -
Associated microRNA: -
Biological function: Supports breast cancer cell viability (knockdown reduces viability).
Molecular mechanism: not specified
Biological pathway or process:

proliferation (promotes); other pathway/process (other)
Detected method:
Q
S
Validation methods:

RNA-seq; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; Transfection; MTT; Survival Analysis
Clinical significance:

Circular, and not linear, CNOT2 levels are predictive for progression-free survival time to aromatase inhibitor (AI) therapy in advanced breast cancer patients; circCNOT2 is detectable in cell-free RNA from plasma.
Description:

circCNOT2 is present in primary breast tumors and detectable in plasma cfRNA. Functional knockdown of circCNOT2 reduces viability of MCF-7 and BT-474 breast cancer cells. In ER-positive advanced breast cancer treated with aromatase inhibitors, higher circCNOT2 levels predict shorter progression-free survival, while linear CNOT2 does not.
Confidence score:

0.7286
Other information
Title:

The circular RNome of primary breast cancer.

Journal: Genome research
Published: 2019
PubMed ID: 30692147
Study type:

combined biological and clinical study
Data availability: EGAS00001001178; GSE5847
Code availability: -