| Expression pattern: |
UP |
| Associated gene: |
CPEB4 |
| Associated microRNA: |
miR-758-3p |
| Biological function: |
promotes cell proliferation, migration, invasion, EMT and tumor growth; knockdown induces G0/G1 cell cycle arrest |
| Molecular mechanism: |
ceRNA/miRNA sponge mechanism: circ_0003221 sponges miR-758-3p to upregulate CPEB4 |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; EdU Staining; Cell Cycle Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
circ_0003221 is up-regulated in cervical cancer tissues and cell lines and functions as an oncogenic circRNA. It promotes proliferation, migration, invasion, EMT and tumor growth by sponging miR-758-3p to increase CPEB4 expression (circ_0003221/miR-758-3p/CPEB4 axis). |
| Confidence score: |
0.7094 |