circRNA basic information
circBase ID: hsa_circ_0078767
Name: hsa_circ_FAM120B
Synonym: circFAM120B
Host Gene: FAM120B
Genomic location(hg19): chr6:170615843-170639638:+
Genomic location(hg38): chr6:170306755-170330550:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: Osteosarcoma
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

primary tumor tissues; paired normal tissues
Cell lines:

MG63; U2OS; 143B; HOS; SJSA-1; hFOB 1.19
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PTBP1, AGO2
Associated microRNA: miR-1205
Biological function: Promotes osteosarcoma cell proliferation and invasion and accelerates tumor growth in vivo.
Molecular mechanism: Acts as a ceRNA/miRNA sponge for miR-1205, thereby increasing PTBP1 expression.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); invasion (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; FISH / smFISH; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; EdU Staining; Transwell Assay; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model
Clinical significance:

Elevated circFAM120B levels were associated with advanced TNM stage and metastasis in osteosarcoma patients.
Description:

circFAM120B (hsa_circ_0078767) is up-regulated in osteosarcoma tissues and cell lines and correlates with advanced TNM stage and metastasis. It promotes osteosarcoma proliferation, colony formation, invasion, and xenograft growth by sponging miR-1205 to increase PTBP1 expression (ceRNA axis).
Confidence score:

0.783
Other information
Title:

CircFAM120B knockdown inhibits osteosarcoma tumorigenesis via the miR-1205/PTBP1 axis.

Journal: Aging
Published: 2021
PubMed ID: 34716285
Study type:

combined biological and clinical study
Data availability: The dataset supporting the conclusions of this article is included within the article.
Code availability: -