| Expression pattern: |
UP |
| Associated gene: |
ITGA6, PI3K, AKT, Ago2 |
| Associated microRNA: |
miR-30e-5p |
| Biological function: |
Promotes NSCLC cell proliferation, migration, invasion and EMT, inhibits apoptosis, and accelerates tumor growth in vivo. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-30e-5p to upregulate ITGA6 and activate the ITGA6/PI3K/AKT axis. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); apoptosis (inhibits); PI3K/AKT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Transfection; CCK8; Colony Formation Assay; TUNEL; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circ-PITX1 up-regulation was correlated with NSCLC patients’ poor survival; high circ-PITX1 associated with higher TNM stage and earlier local lymph node metastasis. |
| Description: |
circ-PITX1 is up-regulated in NSCLC and promotes malignant phenotypes (proliferation, migration/invasion, EMT) while inhibiting apoptosis. It functions mainly as a ceRNA that sponges miR-30e-5p to relieve repression of ITGA6, thereby activating the ITGA6/PI3K/AKT pathway and correlating with poor patient survival. |
| Confidence score: |
0.798 |