| Expression pattern: |
UP |
| Associated gene: |
LDHA, PDK1 |
| Associated microRNA: |
miR-578 |
| Biological function: |
circ-CNST knockdown suppresses proliferation, colony formation, migration, invasion and glycolysis, and promotes apoptosis in osteosarcoma cells; inhibits tumor growth in vivo. |
| Molecular mechanism: |
ceRNA mechanism: circ-CNST sponges miR-578 to regulate LDHA and PDK1 expression, affecting glycolysis and malignant phenotypes. |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis |
| Clinical significance: |
High circ-CNST might predict shorter overall survival, advanced TNM stages, and lymph node metastasis in osteosarcoma patients. |
| Description: |
circ-CNST is up-regulated in osteosarcoma tissues and cell lines and is associated with advanced clinical features and shorter overall survival. Functionally, circ-CNST promotes malignant behaviors and glycolysis by acting as a ceRNA sponge for miR-578, thereby increasing LDHA and PDK1 expression; circ-CNST knockdown suppresses tumor growth in vitro and in a cell line-derived xenograft model. |
| Confidence score: |
0.7482 |