| Expression pattern: |
UP |
| Associated gene: |
BMPR2, Smad5, p38 |
| Associated microRNA: |
miR-6817-5p |
| Biological function: |
promotes osteogenic differentiation of human dental pulp stem cells |
| Molecular mechanism: |
circRFWD2 binds (sponges) miR-6817-5p, relieving repression of BMPR2 and increasing Smad5 and p38 phosphorylation to promote osteogenesis. |
| Biological pathway or process: |
BMP-Smad (promotes); MAPK (promotes); proliferation (other); osteogenesis (other); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Luciferase Reporter Assay; Transfection; Western Blot; CCK8; Clinical Sample Validation |
| Clinical significance: |
suggest potential therapeutic methods for bone defects regeneration |
| Description: |
circRFWD2 is up-regulated during osteogenic induction of human dental pulp stem cells and promotes osteogenic differentiation. It functions as a ceRNA by binding miR-6817-5p, thereby increasing BMPR2 levels and activating Smad5 and p38 phosphorylation (BMP-Smad and p38 MAPK signaling) to enhance osteogenesis. |
| Confidence score: |
0.6099 |