| Expression pattern: |
UP |
| Associated gene: |
MCL1, Ago2 |
| Associated microRNA: |
miR-142-5p |
| Biological function: |
promotes chemoresistance, proliferation, migration, invasion, and tumor growth; inhibits apoptosis; affects cell cycle arrest |
| Molecular mechanism: |
ceRNA sponging miR-142-5p to upregulate MCL1 at the post-transcriptional level |
| Biological pathway or process: |
chemoresistance (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (other); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; FISH / smFISH; Transfection; CCK8; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis; Western Blot; Clinical Sample Validation; Cohort Study |
| Clinical significance: |
high circ_0004674 expression was related to OS patients’ poor prognosis and indicated as an independent prognostic factor |
| Description: |
circ_0004674 (hsa_circ_0004674), derived from ADAM22, is upregulated in chemoresistant osteosarcoma tissues/cell lines and is associated with poor survival. It promotes OS progression and doxorubicin resistance by acting as a cytoplasmic ceRNA that sponges miR-142-5p, thereby relieving repression of MCL1 and increasing its expression. |
| Confidence score: |
0.8921 |