| Expression pattern: |
DN |
| Associated gene: |
CREB, GPX4 |
| Associated microRNA: |
- |
| Biological function: |
CircSCUBE3 inhibits LUAD progression, decreases cell viability and tumorigenesis, and promotes ferroptosis by inhibiting GSH synthesis and downregulating GPX4. |
| Molecular mechanism: |
CircSCUBE3 binds CREB and competitively reduces CREB binding to the GPX4 promoter, thereby suppressing CREB-mediated GPX4 transcription, inhibiting GPX4-mediated GSH synthesis, and promoting ferroptosis. |
| Biological pathway or process: |
ferroptosis (promotes); proliferation (inhibits); mitochondrial function (other); other pathway/process (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; CCK8; ELISA; IF (Immunofluorescence); Flow Cytometry（Non-apoptosis/cycle）; IHC (Immunohistochemistry); RIP (RNA Immunoprecipitation); RNA Pull-Down; ChIP / ChIP-seq; Western Blot; In Vivo Animal Model |
| Clinical significance: |
CircSCUBE3 may provide a novel therapeutic option or candidate target for LUAD treatment. |
| Description: |
circSCUBE3 is downregulated in LUAD tissues and cell lines. Functionally, it suppresses LUAD cell viability and xenograft tumorigenesis by promoting ferroptosis. Mechanistically, circSCUBE3 binds CREB and inhibits CREB-mediated GPX4 transcription, thereby affecting GPX4-mediated GSH synthesis through the CREB/GPX4/GSH axis. |
| Confidence score: |
0.8438 |