circRNA basic information
circBase ID: hsa_circ_0076092
Name: hsa_circ_SCUBE3
Synonym: -
Host Gene: SCUBE3
Genomic location(hg19): chr6:35195356-35201078:+
Genomic location(hg38): chr6:35227579-35233301:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005061
MONDO name: lung adenocarcinoma
Disease details: lung adenocarcinoma
Disease DO ID:
3910
Disease MeSH ID:
C538231
Disease NCIt ID:
C3512
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

LUAD tumor tissues; adjacent normal tissues; mouse tumor tissues

Cell lines:

A549; H1975; BEAS-2B

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
DN
Associated gene: CREB, GPX4
Associated microRNA: -
Biological function: CircSCUBE3 inhibits LUAD progression, decreases cell viability and tumorigenesis, and promotes ferroptosis by inhibiting GSH synthesis and downregulating GPX4.
Molecular mechanism: CircSCUBE3 binds CREB and competitively reduces CREB binding to the GPX4 promoter, thereby suppressing CREB-mediated GPX4 transcription, inhibiting GPX4-mediated GSH synthesis, and promoting ferroptosis.
Biological pathway or process:

ferroptosis (promotes); proliferation (inhibits); mitochondrial function (other); other pathway/process (inhibits)

Detected method:
Q
M
Validation methods:

Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; CCK8; ELISA; IF (Immunofluorescence); Flow Cytometry(Non-apoptosis/cycle); IHC (Immunohistochemistry); RIP (RNA Immunoprecipitation); RNA Pull-Down; ChIP / ChIP-seq; Western Blot; In Vivo Animal Model

Clinical significance:

CircSCUBE3 may provide a novel therapeutic option or candidate target for LUAD treatment.

Description:

circSCUBE3 is downregulated in LUAD tissues and cell lines. Functionally, it suppresses LUAD cell viability and xenograft tumorigenesis by promoting ferroptosis. Mechanistically, circSCUBE3 binds CREB and inhibits CREB-mediated GPX4 transcription, thereby affecting GPX4-mediated GSH synthesis through the CREB/GPX4/GSH axis.

Confidence score:

0.8438

Other information
Title:

CircSCUBE3 promoted ferroptosis to inhibit lung adenocarcinoma progression.

Journal: Cellular and molecular biology (Noisy-le-Grand, France)
Published: 2024
PubMed ID: 38430026
Study type:

combined biological and clinical study

Data availability: GSE101586; GSE158695
Code availability: -