circRNA basic information
circBase ID: -
Name: hsa_circ_GFRA1
Synonym: hsa_circ_005239
Host Gene: GFRA1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0006244
MONDO name: HER2 positive breast carcinoma
Disease details: HER‐2‐positive breast cancer
Disease DO ID:
0060079
Disease MeSH ID:
-
Disease NCIt ID:
C53556
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

breast cancer tissues; paired non‐malignant tissues

Cell lines:

184A; SKBR3; BT474; SW620

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: AIFM2, Ago2
Associated microRNA: miR‐1228
Biological function: Promotes proliferation, invasion/infiltration and metastasis of HER-2-positive breast cancer cells; inhibits ferroptosis.
Molecular mechanism: Acts as a ceRNA/miRNA sponge for miR-1228 to relieve repression of AIFM2, increasing AIFM2 expression and affecting ferroptosis-related pathways.
Biological pathway or process:

proliferation (promotes); invasion (promotes); metastasis (promotes); ferroptosis (inhibits); ceRNA regulation (promotes)

Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Transwell Assay; In Vivo Animal Model; H&E Staining; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; Clinical Sample Validation

Clinical significance:

circGFRA1 is upregulated in HER-2-positive breast cancer tissues and suggested to have prognostic and therapeutic value; proposed as a promising treatment option.

Description:

circGFRA1 (hsa_circ_005239), derived from the GFRA1 gene, is up-regulated in HER-2-positive breast cancer cells and tissues. It promotes malignant behaviors (proliferation, invasion and metastasis) and inhibits ferroptosis by sponging miR-1228 to enhance AIFM2 expression (circGFRA1/miR-1228/AIFM2 ceRNA axis).

Confidence score:

0.8069

Other information
Title:

CircGFRA1 facilitates the malignant progression of HER-2-positive breast cancer via acting as a sponge of miR-1228 and enhancing AIFM2 expression.

Journal: Journal of cellular and molecular medicine
Published: 2021
PubMed ID: 34668628
Study type:

combined biological and clinical study

Data availability: The data that support the fundings of this study are available from the corresponding author upon reasonable request.
Code availability: -