circRNA basic information
circBase ID: hsa_circ_0001368
Name: hsa_circ_KLHL24
Synonym: circKLHL24 / circ_0001368
Host Gene: KLHL24
Genomic location(hg19): chr3:183361267-183390272:+
Genomic location(hg38): chr3:183643479-183672484:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

Breast tumor tissue specimens; matched breast normal tissue specimens; tumor tissues
Cell lines:

BT549; MDA-231; HCC38; SUM-159; MDA-453; MCF10A
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: ALX4
Associated microRNA: miR-1204
Biological function: circKLHL24 suppresses breast cancer cell viability, colony formation, migration, invasion, glycolysis metabolism, and tumor growth in vivo.
Molecular mechanism: circKLHL24 acts as a competing endogenous RNA sponge for miR-1204, thereby upregulating ALX4 and inhibiting breast cancer progression.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); glycolysis (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; In Vivo Animal Model
Clinical significance:

circKLHL24 is proposed as an effective biomarker for breast cancer and the circKLHL24/miR-1204/ALX4 network as a promising strategy for cancer treatment.
Description:

circKLHL24 is downregulated in breast cancer tissues and cell lines. Its overexpression inhibits breast cancer cell viability, proliferation, migration, invasion, glycolysis, and xenograft tumor growth. Mechanistically, circKLHL24 sponges miR-1204 to increase ALX4 expression, forming a circKLHL24/miR-1204/ALX4 tumor-suppressive regulatory network.
Confidence score:

0.8119
Other information
Title:

circKLHL24 Blocks Breast Cancer Development by Regulating the miR-1204/ALX4 Network.

Journal: Cancer biotherapy & radiopharmaceuticals
Published: 2022
PubMed ID: 33781094
Study type:

combined biological and clinical study
Data availability: available from the corresponding author on reasonable request
Code availability: -