| Expression pattern: |
UP |
| Associated gene: |
TOP1, GLI1 promoter |
| Associated microRNA: |
- |
| Biological function: |
Promotes self-renewal maintenance of liver CSCs, tumor initiation and HCC propagation; enhances oncosphere formation and proliferation; circIPO11 knockout/knockdown suppresses liver cancer progression. |
| Molecular mechanism: |
circIPO11 binds and recruits TOP1 to the GLI1 promoter to trigger GLI1 transcription, activating Hedgehog (Hh) signaling; not acting as a miRNA sponge. |
| Biological pathway or process: |
Hedgehog (promotes); stemness (promotes); proliferation (promotes) |
| Detected method: |
Q
B
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RNase R Treatment; Actinomycin D / DRB Stability Assay; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Northern Blot; ISH (In Situ Hybridization); FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; Transwell Assay; Colony Formation Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; ChIP / ChIP-seq; In Vivo Animal Model; H&E Staining; Flow Cytometry（Non-apoptosis/cycle） |
| Clinical significance: |
- |
| Description: |
circIPO11 is up-regulated in HCC tumor tissues and liver CSCs and is required for CSC self-renewal and tumor initiation/propagation. Mechanistically, it binds TOP1 and recruits it to the GLI1 promoter to activate GLI1 transcription, thereby promoting Hedgehog signaling. |
| Confidence score: |
0.8283 |