| Expression pattern: |
UP |
| Associated gene: |
CCL4, CCR5, Ago2 |
| Associated microRNA: |
miR-125a-3p |
| Biological function: |
promotes proliferation, colony formation and invasion; promotes tumor growth in vivo |
| Molecular mechanism: |
acts as a sponge of miR-125a-3p to upregulate the CCL4-CCR5 axis |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
FISH / smFISH; RT-qPCR; divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circ_0002483 expression was associated with TNM stage and poor survival in patients with LUAC |
| Description: |
hsa_circ_0002483 (circ_0002483; circPTK2) is up-regulated in LUAC and correlates with advanced TNM stage and poorer overall survival. Functionally, it promotes LUAC cell proliferation and invasion and enhances xenograft growth. Mechanistically, it sponges miR-125a-3p, relieving repression of the CCL4-CCR5 axis. |
| Confidence score: |
0.8711 |