circRNA basic information
circBase ID: hsa_circ_0044556
Name: hsa_circ_COL1A1
Synonym: circCOL1A1
Host Gene: COL1A1
Genomic location(hg19): chr17:48271490-48272189:-
Genomic location(hg38): chr17:50194129-50194828:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

gastric cancer tissue; adjacent tissues; nude mouse tumor tissue
Cell lines:

MKN-45; SGC-7901; AGS; MGC-803; BGC-823; GES-1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: RABL3
Associated microRNA: miR-145
Biological function: Promotes gastric cancer cell proliferation, migration, and invasion; promotes transformation of M1 into M2 macrophage; promotes tumor growth in vivo.
Molecular mechanism: Acts as a miRNA sponge for miR-145, thereby relieving miR-145-mediated repression of RABL3 (ceRNA axis).
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); macrophage polarization (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Transwell Assay; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; Western Blot; Bioinformatics Analysis
Clinical significance:

-
Description:

circCOL1A1 (hsa_circ_0044556), derived from COL1A1, is up-regulated in gastric cancer tissues and cell lines and mainly localizes to the cytoplasm. It promotes proliferation, migration and invasion, and enhances tumor growth in a cell line-derived xenograft model. Mechanistically, it sponges miR-145 to increase RABL3 expression (circCOL1A1/miR-145/RABL3 axis) and is associated with increased M2 macrophage polarization.
Confidence score:

0.8275
Other information
Title:

circCOL1A1 Promotes the Progression of Gastric Cancer Cells through Sponging miR-145 to Enhance RABL3 Expression.

Journal: Journal of immunology research
Published: 2021
PubMed ID: 34631898
Study type:

combined biological and clinical study
Data availability: The data that support the findings of this study are available from the corresponding author upon reasonable request.
Code availability: -