circRNA basic information
circBase ID: hsa_circ_0003949
Name: hsa_circ_PTN
Synonym: -
Host Gene: PTN
Genomic location(hg19): chr7:136935976-136939721:-
Genomic location(hg38): chr7:137251229-137254974:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0021042
MONDO name: glioma
Disease details: glioma
Disease DO ID:
 -
Disease MeSH ID:
 D005910
Disease NCIt ID:
 C3059
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

glioma tissues; normal brain tissues (NBT)
Cell lines:

U251; U87; LN18; A172; U118; HEB; 293 T; U87-Luc; U87-luc; G15; S15; U87-GSC; U251-GSC
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SOX9, ITGA5
Associated microRNA: miR-145-5p, miR-330-5p
Biological function: promotes glioma cell proliferation and cell cycle progression; promotes tumor growth in vivo; promotes glioma stem cell self-renewal and stemness
Molecular mechanism: acts as a miRNA sponge for miR-145-5p and miR-330-5p; rescues repression of SOX9 and ITGA5 by these miRNAs; promotes stemness via miR-145-5p binding sites
Biological pathway or process:

proliferation (promotes); cell cycle (promotes); stemness (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Sanger Sequencing; Transfection; Luciferase Reporter Assay; RNA Pull-Down; CCK8; EdU Staining; Cell Cycle Assay; Colony Formation Assay; In Vivo Animal Model; Survival Analysis; Clinical Sample Validation; Bioinformatics Analysis
Clinical significance:

-
Description:

circPTN (hsa_circ_0003949), derived from PTN, is up-regulated in glioma and mainly localized in the cytoplasm. It promotes glioma proliferation/cell-cycle progression and in vivo tumor growth by sponging miR-145-5p and miR-330-5p, relieving suppression of SOX9 and ITGA5, and it enhances glioma stem-cell self-renewal and stemness via miR-145-5p binding.
Confidence score:

0.8632
Other information
Title:

circPTN sponges miR-145-5p/miR-330-5p to promote proliferation and stemness in glioma.

Journal: Journal of experimental & clinical cancer research : CR
Published: 2019
PubMed ID: 31511040
Study type:

combined biological and clinical study
Data availability: available from the corresponding author on reasonable request
Code availability: -