circRNA basic information
circBase ID: hsa_circ_0024093
Name: hsa_circ_YAP1
Synonym: -
Host Gene: YAP1
Genomic location(hg19): chr11:101981191-102104154:+
Genomic location(hg38): chr11:102110460-102233423:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0006659
MONDO name: arteriosclerosis obliterans
Disease details: Arteriosclerosis obliterans (ASO) of the lower extremities
Disease DO ID:
 5160
Disease MeSH ID:
 D001162
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

VSMCs
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: USP9X, YAP1, Ago2
Associated microRNA: miR-889-3p, miR-4677-3p
Biological function: Promotes proliferation, promotes migration, and inhibits apoptosis in PDGF-BB-treated VSMCs (in vitro ASO model).
Molecular mechanism: Acts as a ceRNA to sponge miR-889-3p/miR-4677-3p, upregulating USP9X, which induces YAP1 deubiquitination and stabilizes YAP1 protein.
Biological pathway or process:

proliferation (promotes); migration (promotes); apoptosis (inhibits); ubiquitination (inhibits); ceRNA regulation (promotes); Hippo/YAP (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Western Blot; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; EdU Staining; CCK8; TUNEL; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Co-IP
Clinical significance:

-
Description:

In a PDGF-BB-induced in vitro model relevant to lower-extremity ASO, hsa_circ_0024093 is upregulated and promotes VSMC proliferation and migration while inhibiting apoptosis. It functions mainly in the cytoplasm as a ceRNA that sponges miR-889-3p and miR-4677-3p to increase USP9X, which deubiquitinates and stabilizes YAP1 protein.
Confidence score:

0.6909
Other information
Title:

Hsa_circ_0024093 accelerates VSMC proliferation via miR-4677-3p/miR-889-3p/USP9X/YAP1 axis in in vitro model of lower extremity ASO.

Journal: Molecular therapy. Nucleic acids
Published: 2021
PubMed ID: 34631281
Study type:

biological research
Data availability: Supplemental information can be found online at https://doi.org/10.1016/j.omtn.2021.07.026.
Code availability: -