| Expression pattern: |
UP |
| Associated gene: |
METRN |
| Associated microRNA: |
miR-615 |
| Biological function: |
promotes retinal reactive gliosis and glial cell activation, and impairs RGC survival during glaucoma-induced retinal neurodegeneration; promotes Müller cell viability and proliferation |
| Molecular mechanism: |
Acts as an endogenous miR-615 sponge to inhibit miR-615 activity, leading to increased METRN; METRN overexpression partially rescues effects of cZNF609 silencing on glial proliferation |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Sanger Sequencing; RNase R Treatment; Clinical Sample Validation; Transfection; RNA Pull-Down; Luciferase Reporter Assay; MTT; IF (Immunofluorescence); Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
In rat glaucoma models, cZNF609 is up-regulated and promotes retinal neurodegeneration by enhancing reactive gliosis and Müller glial activation, thereby impairing RGC survival. Mechanistically, cZNF609 functions as a ceRNA that sponges miR-615 to derepress METRN, supporting glial proliferation/activation. |
| Confidence score: |
0.7782 |