| Expression pattern: |
UP |
| Associated gene: |
SLC16A9, CDH1, CDH2, VIM, E-cadherin, N-cadherin, Vimentin |
| Associated microRNA: |
miR-1294 |
| Biological function: |
circPLK1 promotes NSCLC cell viability, proliferation, migration, invasion and tumor growth, and inhibits apoptosis; silencing circPLK1 suppresses malignant progression. |
| Molecular mechanism: |
circPLK1 acts as a ceRNA sponge for miR-1294 and promotes SLC16A9 expression, thereby regulating the miR-1294/SLC16A9 axis in NSCLC. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); EMT (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
The circPLK1-miR-1294-SLC16A9 axis holds promise as a novel therapeutic target and potential biomarker for the clinical diagnosis of NSCLC. |
| Description: |
circPLK1 is up-regulated in NSCLC cells and functions as an oncogenic circRNA. Its knockdown suppresses proliferation, viability, migration, invasion and xenograft tumor growth while promoting apoptosis. Mechanistically, circPLK1 sponges miR-1294 to promote SLC16A9 expression, forming a circPLK1/miR-1294/SLC16A9 regulatory axis with potential therapeutic and biomarker relevance. |
| Confidence score: |
0.5634 |