| Expression pattern: |
UP |
| Associated gene: |
mTOR, Ago2 |
| Associated microRNA: |
miR-144 |
| Biological function: |
Promotes NSCLC proliferation, migration, invasion, tumor growth and metastasis; facilitates EMT and inhibits MET. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-144, thereby regulating mTOR and AKT/mTOR signaling. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); PI3K/AKT/mTOR (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Nuclear-Cytoplasmic Fractionation; divergent primers PCR; RNase R Treatment; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
High circDLG1 expression is associated with lower survival rates/poorer prognosis and with lymph node metastasis, tumor stages, and distant metastasis. |
| Description: |
circDLG1 is up-regulated in NSCLC tissues and cell lines and functions as an oncogenic circRNA. It sponges miR-144 to elevate mTOR and activate AKT/mTOR signaling, thereby promoting proliferation, migration/invasion, tumor growth, and EMT-related metastatic traits; high circDLG1 is associated with poor patient survival. |
| Confidence score: |
0.8632 |