circRNA basic information
circBase ID: -
Name: hsa_circ_CRKL
Synonym: circCRKL
Host Gene: CRKL
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0018874
MONDO name: acute myeloid leukemia
Disease details: acute myeloid leukemia
Disease DO ID:
 9119
Disease MeSH ID:
 D015470
Disease NCIt ID:
 C3171
Disease ICD11 ID:
 -
Disease OMIM ID:
 601626
Species: Human
Species details: Homo sapiens
Tissue specimen:

bone marrow (BM) samples
Cell lines:

KG-1a; KG-1; Kasumi-1; THP1; MOLM13; HS-5
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: p27
Associated microRNA: miR-196a-5p, miR-196b-5p
Biological function: inhibits proliferation; inhibits colony-forming ability; induces cell cycle arrest
Molecular mechanism: ceRNA sponge of miR-196a-5p and miR-196b-5p to increase p27 expression
Biological pathway or process:

proliferation (inhibits); cell cycle (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; RIP (RNA Immunoprecipitation); circRIP; RNA Pull-Down; Luciferase Reporter Assay; Western Blot; Clinical Sample Validation; Bioinformatics Analysis
Clinical significance:

potential therapeutic target for AML
Description:

circCRKL is down-regulated in acute myeloid leukemia and functions as a tumor-suppressive circRNA by inhibiting AML cell proliferation, colony formation, and cell-cycle progression. Mechanistically, it acts as a ceRNA that sponges miR-196a-5p and miR-196b-5p, thereby relieving repression of p27 (CDKN1B) and promoting p27 expression.
Confidence score:

0.8043
Other information
Title:

Circular RNA circCRKL inhibits the proliferation of acute myeloid leukemia cells via the miR-196a-5p/miR-196b-5p/p27 axis.

Journal: Bioengineered
Published: 2021
PubMed ID: 34617876
Study type:

combined biological and clinical study
Data availability: Supplemental data for this article can be accessed here
Code availability: -