| Expression pattern: |
DN |
| Associated gene: |
Smad2, Ago2 |
| Associated microRNA: |
miR-125a-5p |
| Biological function: |
regulates ECM metabolism, inhibits apoptosis, and promotes proliferation in chondrocytes; alleviates osteoarthritis |
| Molecular mechanism: |
CircCDK14 acts as a miRNA sponge for miR-125a-5p to relieve repression of Smad2 and modulate TGF-beta signaling in osteoarthritis |
| Biological pathway or process: |
TGF-beta/SMAD (promotes); apoptosis (inhibits); proliferation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; FISH / smFISH; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Western Blot; IF (Immunofluorescence); EdU Staining; Annexin V/PI Flow Cytometry; In Vivo Animal Model; IHC (Immunohistochemistry); TUNEL; H&E Staining |
| Clinical significance: |
- |
| Description: |
CircCDK14 is down-regulated in osteoarthritic weight-bearing cartilage and exerts protective effects by maintaining ECM anabolism, promoting proliferation, and inhibiting apoptosis in chondrocytes. Mechanistically, it functions as a cytoplasmic ceRNA that sponges miR-125a-5p to support Smad2 expression and modulate TGF-beta/SMAD signaling; AAV-mediated CircCDK14 overexpression alleviates OA in an ACLT rabbit model. |
| Confidence score: |
0.8378 |