| Expression pattern: |
UP |
| Associated gene: |
WNT5B, beta-catenin |
| Associated microRNA: |
miR-7-5p |
| Biological function: |
promotes adipogenic differentiation and suppresses osteogenic differentiation of BMSCs in SONFH |
| Molecular mechanism: |
ceRNA mechanism by sponging miR-7-5p to regulate WNT5B and downstream beta-catenin signaling |
| Biological pathway or process: |
Wnt/beta-catenin (inhibits); adipogenesis (promotes); osteogenesis (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Luciferase Reporter Assay; Transfection; Western Blot; Oil Red O Staining; Alizarin Red S (ARS) Staining |
| Clinical significance: |
new biomarkers for the diagnosis and treatment of SONFH |
| Description: |
CDR1as is significantly up-regulated in SONFH-BMSCs and functions as a miR-7-5p sponge to increase WNT5B expression. This promotes adipogenic differentiation and suppresses osteogenic differentiation, likely through the WNT5B/beta-catenin-related pathway, making CDR1as a potential diagnostic and therapeutic biomarker for SONFH. |
| Confidence score: |
0.5627 |