| Expression pattern: |
UP |
| Associated gene: |
PBX3, EIF4A3, AGO2 |
| Associated microRNA: |
miR-516a-5p |
| Biological function: |
promotes proliferation and metastasis |
| Molecular mechanism: |
EIF4A3 promotes circTOLLIP biogenesis; circTOLLIP sponges miR-516a-5p to elevate PBX3 expression and activate EMT |
| Biological pathway or process: |
proliferation (promotes); metastasis (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; ISH (In Situ Hybridization); Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; RNA-seq; In Vivo Animal Model; H&E Staining; Survival Analysis; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
High circTOLLIP expression was correlated with poor overall survival (OS) and disease-free survival (DFS) in patients. |
| Description: |
circTOLLIP (hsa_circ_0008301), derived from TOLLIP, is up-regulated in hepatocellular carcinoma and associated with poor OS/DFS. It promotes HCC proliferation and metastasis by acting as a ceRNA that sponges miR-516a-5p to increase PBX3 and activate EMT, while its biogenesis is enhanced by the RBP EIF4A3. |
| Confidence score: |
0.8944 |