| Expression pattern: |
UP |
| Associated gene: |
PD-L1, AKT, p-AKT |
| Associated microRNA: |
miR-145 |
| Biological function: |
promotes EMT, migration, invasion, and in vivo tumor growth in HCC |
| Molecular mechanism: |
Acts as a miR-145 sponge to upregulate PD-L1 and activate PI3K/Akt signaling, thereby promoting EMT and invasion |
| Biological pathway or process: |
EMT (promotes); invasion (promotes); migration (promotes); metastasis (promotes); PI3K/AKT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Luciferase Reporter Assay; RNA Pull-Down; FISH / smFISH; Transfection; Transwell Assay; Western Blot; Clinical Sample Validation; In Vivo Animal Model |
| Clinical significance: |
High hsa_circ_0003288 expression was reported (in a previous study) to be associated with poor prognosis in HCC; in this study, hsa_circ_0003288 was increased in HCC tissues and positively correlated with PD-L1 expression. |
| Description: |
hsa_circ_0003288 is up-regulated in HCC tissues/cell lines and mainly localized in the cytoplasm. It promotes EMT, migration and invasion by sponging miR-145 to de-repress PD-L1, with downstream activation of the PI3K/Akt pathway; knockdown suppresses tumor growth and EMT in xenograft models. |
| Confidence score: |
0.7046 |