| Expression pattern: |
UP |
| Associated gene: |
STC2, SP3, Ago2 |
| Associated microRNA: |
miR-361-3p |
| Biological function: |
Promotes proliferation and tumor growth in hepatocellular carcinoma; knockdown inhibits proliferation and colony formation in vitro and suppresses tumor-forming ability in vivo. |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-361-3p to increase STC2 expression; circ_0011385 transcription is activated by SP3. |
| Biological pathway or process: |
proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; In Vivo Animal Model; ChIP / ChIP-seq; Western Blot; Microarray |
| Clinical significance: |
High circ_0011385 expression was associated with tumor size, TNM stage, and poorer prognosis in HCC patients; proposed as a biomarker for diagnosis and treatment. |
| Description: |
circ_0011385 is up-regulated in HCC and promotes HCC cell proliferation and xenograft tumor growth. Mechanistically, it functions mainly in the cytoplasm as a ceRNA sponging miR-361-3p to upregulate STC2, and its transcription is activated by SP3. High circ_0011385 expression correlates with more aggressive clinicopathologic features and poorer survival. |
| Confidence score: |
0.8547 |