circRNA basic information
circBase ID: -
Name: -
Synonym: HBV_circ_1
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: HBV-related hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Virus
Species details: Hepatitis B virus
Tissue specimen:

HBV-related hepatocellular carcinoma tissue (HCCT); paracancerous tissues; HCC tumor specimens; paired paracancerous tissues; tumor tissue
Cell lines:

HepG2.2.15; HepG2; SMCC-7721; AML-121
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: CDK1, CDK18, PHB, U2AF35, SRSF1, RACK1, ANXA2, SRSF2, EEF1A1, SNRPB2, ZNF26, EXOSC6, MAPKAP1
Associated microRNA: -
Biological function: promotes cell proliferation, migration, and invasion; inhibits apoptosis; promotes tumor growth
Molecular mechanism: HBV_circ_1 directly binds CDK1, co-localizes with CDK1, and increases CDK1 expression to promote HCC progression-related phenotypes
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes)
Detected method:
Q
B
H
S
M
Validation methods:

divergent primers PCR; RNase R Treatment; Sanger Sequencing; RNA-seq; Microarray; Northern Blot; RT-qPCR; ISH (In Situ Hybridization); FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; MTT; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis
Clinical significance:

HBV_circ_1 abundance was higher in HCCT than in paracancerous tissues; HBV_circ_1-positive patients had lower survival rate
Description:

HBV-encoded HBV_circ_1 is up-regulated in HBV-related HCC tissues and is associated with worse patient survival. Functional assays indicate HBV_circ_1 promotes proliferation, cell-cycle progression, migration/invasion and tumor growth while inhibiting apoptosis, mainly by directly binding to CDK1 and increasing CDK1 expression.
Confidence score:

0.8536
Other information
Title:

Hepatocellular carcinoma progression mediated by hepatitis B virus-encoded circRNA HBV_circ_1 through interaction with CDK1.

Journal: Molecular therapy. Nucleic acids
Published: 2021
PubMed ID: 34589285
Study type:

combined biological and clinical study
Data availability: SRR7812043-SRR7812048; SRR7892713-SRR7892718
Code availability: -