circRNA basic information
circBase ID: -
Name: hsa_circ_CCDC66
Synonym: circCCDC66
Host Gene: CCDC66
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; corresponding normal tissues
Cell lines:

HCT116; SW620; NCM460; 293T
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: Beclin1, p62, Bax, Bcl-2
Associated microRNA: miR-3140
Biological function: Promotes viability/proliferation, migration and invasion and inhibits apoptosis of CRC cells, including under hypoxic conditions.
Molecular mechanism: circCCDC66 acts as a ceRNA/miRNA sponge for miR-3140, thereby promoting autophagy and CRC malignant phenotypes under hypoxia.
Biological pathway or process:

autophagy (promotes); apoptosis (inhibits); proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis
Clinical significance:

-
Description:

circCCDC66 is upregulated in colorectal cancer tissues/cell lines and further induced by hypoxia. Functionally, circCCDC66 promotes CRC cell viability, migration and invasion and suppresses apoptosis under hypoxic conditions by sponging miR-3140, thereby relieving miR-3140-mediated inhibition of autophagy.
Confidence score:

0.6536
Other information
Title:

Hypoxia‑induced circCCDC66 promotes the tumorigenesis of colorectal cancer via the miR‑3140/autophagy pathway.

Journal: International journal of molecular medicine
Published: 2020
PubMed ID: 33125087
Study type:

combined biological and clinical study
Data availability: The datasets used and/or analyzed during the present study are available from the corresponding author on reasonable request.
Code availability: -