| Expression pattern: |
DN |
| Associated gene: |
PAQR3, AGO2 |
| Associated microRNA: |
miR-203a-3p |
| Biological function: |
tumor suppressor that inhibits tumor growth and metastasis, suppresses proliferation, migration, invasion, and EMT in cervical cancer |
| Molecular mechanism: |
ceRNA sponging miR-203a-3p to restore PAQR3 expression, affecting AKT and ERK signaling and EMT markers |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); EMT (inhibits); PI3K/AKT (inhibits); ERK (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; ISH (In Situ Hybridization); RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Survival Analysis |
| Clinical significance: |
Lower expression of hsa_circ_0043280 is correlated with poor prognosis (poor OS and DFS) and is an independent prognostic factor in CCa. |
| Description: |
hsa_circ_0043280 (from TADA2A) is down-regulated in cervical cancer and acts as a tumor suppressor. It sponges miR-203a-3p to restore PAQR3 expression, thereby inhibiting proliferation, migration/invasion, EMT and metastasis, with downstream suppression of AKT and ERK signaling. |
| Confidence score: |
0.8826 |