| Expression pattern: |
UP |
| Associated gene: |
MMP-16, Ago2, EXOs |
| Associated microRNA: |
miR-375 |
| Biological function: |
promotes invasion and migration (metastasis-related phenotypes) of HCC cells; associated with tumor growth and lung metastasis in vivo |
| Molecular mechanism: |
Acts as a miR-375 sponge (ceRNA), leading to upregulation of MMP-16; exosome-mediated transfer/regulation in HCC |
| Biological pathway or process: |
migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Transwell Assay; Wound Healing Assay; Western Blot; Clinical Sample Validation; Cohort Study; Survival Analysis; ROC Analysis; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining |
| Clinical significance: |
High circ-0072088 is associated with larger tumor size and higher TNM stage; shows high diagnostic value (AUC=0.899); high expression predicts unfavorable prognosis and decreased 5-year survival; independent prognostic factor |
| Description: |
circ-0072088 is up-regulated in HCC tumor tissues and circulating serum/plasma exosomes, and higher levels associate with advanced clinicopathologic features and worse survival. Mechanistically, it localizes mainly to the cytoplasm and acts as a ceRNA sponging miR-375 to relieve repression of MMP-16, thereby promoting EMT-like changes and enhancing HCC cell migration/invasion; exosome-mediated transfer of circ-0072088 modulates metastasis and tumor growth in vivo. |
| Confidence score: |
0.8087 |