circRNA basic information
circBase ID: hsa_circ_0000073
Name: -
Synonym: -
Host Gene: -
Genomic location(hg19): chr1:58992932-59002413:-
Genomic location(hg38): chr1:58527260-58536741:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues
Cell lines:

hFOB1.19; MG63; U-2; Saos2; U2OS; HEK-293; MG-63; Saos-2; HEK293
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: CCNE2, MDM2, Ago2
Associated microRNA: miR-1252-5p
Biological function: promotes proliferation, migration and invasion; promotes tumorigenesis; regulates cell cycle
Molecular mechanism: acts as a miRNA sponge (ceRNA) for miR-1252-5p to regulate CCNE2 and MDM2
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); metastasis (other); PI3K/AKT (other); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Bioinformatics Analysis; Microarray; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Cell Cycle Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

-
Description:

hsa_circ_0000073 is up-regulated in osteosarcoma cell lines and promotes malignant behaviors (proliferation, migration, invasion, cell-cycle progression) and tumorigenesis. Mechanistically, it functions as a ceRNA by sponging miR-1252-5p to regulate CCNE2 and MDM2 expression, supported by luciferase, RIP, and rescue experiments.
Confidence score:

0.8124
Other information
Title:

Circular RNA hsa_circ_0000073 Enhances Osteosarcoma Cells Malignant Behavior by Sponging miR-1252-5p and Modulating CCNE2 and MDM2.

Journal: Frontiers in cell and developmental biology
Published: 2021
PubMed ID: 34568326
Study type:

combined biological and bioinformatics study
Data availability: GSE176373; GSE96964
Code availability: -