| Expression pattern: |
UP |
| Associated gene: |
IRF4, C/EBP-delta |
| Associated microRNA: |
let-7c |
| Biological function: |
promotes abdominal aortic aneurysm formation by promoting M1 macrophage polarization and M1-type inflammation; promotes vascular inflammation |
| Molecular mechanism: |
binds IRF4 and inhibits IRF4 nuclear entry; ceRNA/miRNA sponge mechanism via let-7c to increase C/EBP-delta expression |
| Biological pathway or process: |
macrophage polarization (promotes); inflammation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; ISH (In Situ Hybridization); Clinical Sample Validation; Transfection; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot; Flow Cytometry（Non-apoptosis/cycle）; IHC (Immunohistochemistry); ELISA; In Vivo Animal Model; H&E Staining |
| Clinical significance: |
circCdyl is upregulated in human AAA tissues and may be a therapeutic target for AAA treatment |
| Description: |
circCdyl is up-regulated in mouse and human AAA tissues and promotes AAA formation by enhancing M1 macrophage polarization and M1-type inflammation. Mechanistically, it binds IRF4 to block its nuclear entry and also acts as a let-7c sponge to increase C/EBP-delta expression in macrophages. |
| Confidence score: |
0.8726 |