| Expression pattern: |
UP |
| Associated gene: |
TCF12, AGO2 |
| Associated microRNA: |
miR-211-5p |
| Biological function: |
promotes proliferation, migration, and invasion; promotes tumor growth and metastasis; promotes EMT |
| Molecular mechanism: |
circUSP10 functions as a ceRNA/miRNA sponge for miR-211-5p to regulate TCF12 and promote EMT signaling. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Sanger Sequencing; RNase R Treatment; divergent primers PCR; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circUSP10 expression correlated with tumor size and TNM stage, and high circUSP10 levels corresponded to shorter OS; suggested as a diagnostic and prognostic biomarker for LC. |
| Description: |
circUSP10 is upregulated in liver cancer and promotes proliferation, migration, invasion, and tumor growth. It mainly localizes to the cytoplasm and acts as a ceRNA by sponging miR-211-5p, thereby upregulating TCF12 and activating EMT-associated changes. High circUSP10 expression correlates with advanced clinicopathological features and poorer overall survival. |
| Confidence score: |
0.8769 |