circRNA basic information
circBase ID: hsa_circ_0067842
Name: hsa_circ_SMC4
Synonym: -
Host Gene: SMC4
Genomic location(hg19): chr3:160138527-160146730:+
Genomic location(hg38): chr3:160420739-160428942:+
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BC tissues; adjacent normal breast tissues
Cell lines:

HEK-293T; MCF-10 A; MDA-MB-231; MDA-MB-468; MCF-7; BT549; T-47D
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: HuR, CMTM6, PD-L1
Associated microRNA: -
Biological function: Promotes migration and invasion (metastasis-related behaviors) and promotes immune escape of breast cancer cells.
Molecular mechanism: Binds HuR and promotes its nuclear-to-cytoplasmic translocation, thereby enhancing CMTM6 mRNA stability; increased CMTM6 decreases PD-L1 ubiquitination and inhibits PD-L1 degradation, contributing to immune escape.
Biological pathway or process:

migration (promotes); invasion (promotes); metastasis (promotes); immune regulation (promotes); ubiquitination (inhibits); mRNA stability (promotes)
Detected method:
Q
H
M
Validation methods:

Microarray; RT-qPCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; ISH (In Situ Hybridization); Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; IF (Immunofluorescence); Co-IP; Flow Cytometry（Non-apoptosis/cycle）; Clinical Sample Validation; Cohort Study; Survival Analysis
Clinical significance:

High hsa_circ_0067842 expression is significantly associated with short disease-free survival (DFS) and overall survival (OS) and serves as an independent prognostic factor for BC patients.
Description:

hsa_circ_0067842 is upregulated in breast cancer and is associated with worse survival. It promotes migration/invasion and immune escape by binding the RBP HuR, facilitating HuR cytoplasmic translocation to stabilize CMTM6 mRNA, which in turn reduces PD-L1 ubiquitination and inhibits PD-L1 degradation.
Confidence score:

0.8818
Other information
Title:

Circular RNA hsa_circ_0067842 facilitates tumor metastasis and immune escape in breast cancer through HuR/CMTM6/PD-L1 axis.

Journal: Biology direct
Published: 2023
PubMed ID: 37592296
Study type:

combined biological and clinical study
Data availability: The online version contains supplementary material available at 10.1186/s13062-023-00397-3.; The datasets used and/or analyzed during the current study are available from the corresponding authors on reasonable request.
Code availability: -