| Expression pattern: |
UP |
| Associated gene: |
RPN2, CREB-binding protein (CREBBP), Ago2 |
| Associated microRNA: |
miR-130a-5p |
| Biological function: |
Circ_0037655 promotes glioma malignant behaviors and counteracts sevoflurane-induced inhibition of cell viability, proliferation, migration, invasion and EMT, while inhibiting apoptosis. |
| Molecular mechanism: |
Circ_0037655 functions as a miR-130a-5p sponge to upregulate RPN2, thereby modulating the anti-tumor response to sevoflurane in glioma. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
Circ_0037655 is up-regulated in glioma tissues and cell lines, and sevoflurane treatment suppresses its expression. Functionally, circ_0037655 overexpression weakens the anti-tumor effects of sevoflurane by restoring glioma cell proliferation, migration, invasion and EMT while reducing apoptosis. Mechanistically, circ_0037655 acts through the circ_0037655/miR-130a-5p/RPN2 axis to promote RPN2 expression. |
| Confidence score: |
0.7296 |