| Expression pattern: |
DN |
| Associated gene: |
ZEB2 |
| Associated microRNA: |
miR-218-5p |
| Biological function: |
CircTMCO3 alleviated sepsis-induced acute kidney injury by improving renal function, ameliorating tubular injury, and decreasing apoptosis, oxidative stress and inflammation. |
| Molecular mechanism: |
CircTMCO3 acts as a miRNA sponge for miR-218-5p and regulates ZEB2 through the miR-218-5p/ZEB2 axis. |
| Biological pathway or process: |
apoptosis (inhibits); inflammation (inhibits); other pathway/process (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Sanger Sequencing; RNase R Treatment; RT-qPCR; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; Annexin V/PI Flow Cytometry; ELISA; Western Blot; In Vivo Animal Model; H&E Staining; TUNEL |
| Clinical significance: |
- |
| Description: |
CircTMCO3 is down-regulated in LPS-induced HK-2 cells and CLP-induced mouse kidney tissues. Its overexpression protects against S-AKI by reducing apoptosis, oxidative stress and inflammatory cytokine production, acting through a circTMCO3/miR-218-5p/ZEB2 regulatory axis. |
| Confidence score: |
0.6964 |