| Expression pattern: |
UP |
| Associated gene: |
GREM1 |
| Associated microRNA: |
miR-183-5p |
| Biological function: |
circ_DHRS3 knockdown promoted IL-1beta-impaired chondrocyte proliferation and alleviated IL-1beta-induced apoptosis and extracellular matrix degradation, suggesting that elevated circ_DHRS3 contributes to chondrocyte injury in OA models. |
| Molecular mechanism: |
circ_DHRS3 acts as a competing endogenous RNA that sponges miR-183-5p, thereby protecting GREM1 from miR-183-5p-mediated repression and positively regulating GREM1 expression. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (inhibits); apoptosis (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Transfection; MTT; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circ_DHRS3 is up-regulated in osteoarthritis cartilage tissues and IL-1beta-treated human chondrocytes. Functionally, circ_DHRS3 promotes OA-like chondrocyte injury by suppressing proliferation and enhancing apoptosis and ECM degradation through a circ_DHRS3/miR-183-5p/GREM1 ceRNA axis. |
| Confidence score: |
0.7551 |