| Expression pattern: |
UP |
| Associated gene: |
CD73, GAL-8 |
| Associated microRNA: |
miR-1236-3p |
| Biological function: |
Promotes ICC progression by enhancing proliferation, invasion and metastasis; induces immunosuppressive tumor microenvironment and anti-PD1 resistance. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-1236-3p, thereby upregulating CD73 and GAL-8 to suppress T cell proliferation/infiltration and promote immune escape and anti-PD1 resistance. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); metastasis (promotes); immune regulation (inhibits); ceRNA regulation (promotes); drug resistance (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; ISH (In Situ Hybridization); Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; FISH / smFISH; circRIP; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Western Blot; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); ELISA; Flow Cytometry（Non-apoptosis/cycle）; Cohort Study; Survival Analysis; Bioinformatics Analysis; RNA-seq |
| Clinical significance: |
High circHMGCS1-016 level in tumor tissues is associated with shorter survival and higher recurrence after resection; patients with high levels benefit less from anti-PD1 treatment. |
| Description: |
circHMGCS1-016 (hsa_circ_0008621), derived from HMGCS1, is up-regulated in ICC tissues and promotes ICC cell proliferation, invasion and metastasis. Mechanistically, it sponges miR-1236-3p to increase CD73 and GAL-8, leading to an immunosuppressive microenvironment with reduced T cell activity and decreased responsiveness to anti-PD1 therapy. High circHMGCS1-016 in tumors is associated with poorer survival and higher recurrence. |
| Confidence score: |
0.8933 |